Genomic Sequencing

A collection of 26 posts

Paid-members only

Exomes

Element Bioscience makes a big bet on rapid exome sequencing

RNA

Don't let highly expressed transcripts get the best of your RNA-seq dataset

Whole transcriptome sequencing AKA RNA-seq: The good, the bad, the dynamic range?

Paid-members only

Genomic Sequencing

After 13 years, Illumina's 'little sequencer that could' has been replaced by a bigger, faster sibling

High-Throughput Sequencing

Checking your metrics is an important piece of the sequencing process

High throughput sequencing metrics: Don't be a monster, review them before sending data to the triage team.

Genomic Sequencing

Son of a Bias, why does my sequencing dataset look like trash?

High throughput sequencing: Biases and how they can get the best of you and your data

Paid-members only

Start-Upomics

Nabsys is back, baby!

High-Throughput Sequencing

Being accurate about sequencing accuracy

High throughput sequencing metrics: Let’s be accurate about accuracy.

Paid-members only

Genomic Sequencing

Oxford Nanopore says that the short-read vs long-read conversation is pointless because they can do both

DNA Featured

What came first, DNA methylation or the variant?

DNA sequence variants can change more than just the properties of the proteins that genes code for, they can also change what proteins get expressed!

Genomic Sequencing

Everything you ever wanted to know about cluster based and single-molecule sequencing

Clusters vs single molecules in DNA sequencing: Here’s the short and the long of it.

Genomics

Why short-read sequencing can't ever get us a 'whole genome'

Short-reads miss things, or: How I learned to stop worrying and love the long-read.

Genomic Sequencing

Frederick Sanger sequenced the first DNA genome, he didn't use 'Sanger Sequencing' to do it

Frederick Sanger invented a famous DNA sequencing method. It's not the one pictured below (which he also invented).

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Start-Upomics

Dual barcodes spark a duel between Molecular Loop and Illumina

Genomic Sequencing

Counting the things short-reads are good for

What's the point of short-reads if long-reads do basically everything in genomics better? There are five, FIVE, *thunderclap*, applications where short reads excel, ah, ah, ah!

Genomic Sequencing

Sequencing libraries and strategies for doing quality control

You can't do sequencing without libraries. But you don't know if you have libraries unless you look at them.

RNA

Bridge RNAs are here to take CRISPR's crown

Move over CRISPR, bridge RNAs are here to make the precision genome edits that you can't.

Paid-members only

Start-Upomics

Who's prioritizing bringing the next generation of sequencing technology to the clinic? The answer might surprise you.

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Genomic Sequencing

Spatial omics (and other creative applications) might give that dying NovaSeq of yours a second life!

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Genomic Sequencing Featured

Quest Diagnostics has made some moves in an attempt to become a major player in high throughput genomics

Genomic Sequencing

Commercial industrial engineering is the savior of high-throughput genomics

We ‘completed’ the human genome in 2003, thanks mostly to commercial industrial engineering.

Paid-members only

Genomic Sequencing Featured

Illumina ruffled some feathers this week with a video appeal to moderate sized "core labs"

Genomic Sequencing

The human genome sequence is finally (almost) totally complete. The pesky Y chromosome was the last hold-out!

The human Y-chromosome has finally been fully sequenced! No, seriously this time, the genome is actually finished now, maybe.

Proteomics

Protein sequencing is the latest cutting edge technique in our proteomics toolkit

Protein sequencing is the latest cutting edge technique in our proteomics toolkit. But when will it be ready for primetime?

Genomic Sequencing

Why Transcriptomes are sometimes more useful than Whole Genomes

Transcriptomes: Underappreciated, underutilized, and sometimes more useful than WGS.

Paid-members only

Genomics Featured

With the FDA setting up camp in LDT-ville, which sequencing companies are best suited to help labs transition to IVDs?